| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | MITCHELL, John | SO | Francis Howell High School | NT | Unverified: |
| 4 | SUN, Samuel | JR | Clayton High School | NT | Unverified: |
| 5 | UDODOR, Josiah | JR | Metro High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | SULTAN, Blnd | FR | Metro High School | NT | Unverified: |
| 3 | COHEN, Tae'Vaeun | FR | Francis Howell High School | NT | Unverified: |
| 4 | JOHNSON, Aiden | FR | Francis Howell High School | 1:04.38 | Verified |
| 5 | MOEHLENBROCK, Bailey | JR | Affton High School | NT | |
| 6 | LONG, Linden | FR | Metro High School | NT | Unverified: |
| 7 | MORALES, Kevin | FR | Affton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | IHRIG, Ryan | SO | Francis Howell High School | 1:03.34 | Verified |
| 2 | MARTIN, Merrimon | FR | Jennings High School | 1:02.92 | Verified |
| 3 | FLOOD, Jackson | SO | Francis Howell High School | 1:01.43 | Verified |
| 4 | LYTLE, Joseph | JR | Francis Howell High School | 59.34 | Verified |
| 5 | BELKO, William | SO | Francis Howell High School | 1:01.34 | Verified |
| 6 | LAMARTINA, Logan | JR | Clayton High School | 1:02.19 | Verified |
| 7 | KEOSHKERIAN, Benjamin | SO | Metro High School | 1:03.17 | Verified |
| 8 | WINSTON, Percy | SO | Francis Howell High School | 1:03.54 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUYETTE, Logan | SO | Clayton High School | 57.46 | Verified |
| 2 | PASHKOV, Christopher | SR | Affton High School | 57.15 | Verified |
| 3 | WILLIAMS, Reid | SO | Francis Howell High School | 56.50 | Unverified: |
| 4 | WITT, Curtiss | FR | Francis Howell High School | 56.00 | Unverified: |
| 5 | HENRY, Anthony | SO | Francis Howell High School | 56.00 | Unverified: |
| 6 | MOORE, Camden | SO | Francis Howell High School | 57.00 | Unverified: |
| 7 | HORTHORN, Thajay | FR | Jennings High School | 57.42 | Verified |
| 8 | ABU-ELAWI, Adam | SO | Francis Howell High School | 59.04 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FOREMAN, Tomas | SO | Francis Howell High School | 55.00 | Unverified: |
| 2 | MILLER, Carson | JR | Francis Howell High School | 53.61 | Verified |
| 3 | SEZEN, Aydin | SO | Francis Howell High School | 53.52 | Verified |
| 4 | MARLEY, Jaydon | JR | Metro High School | 51.14 | Verified |
| 5 | GURLEY, Taj | SR | Francis Howell High School | 52.22 | Verified |
| 6 | MAIS, Addison | SO | Francis Howell High School | 53.56 | Verified |
| 7 | DEAL, Joshua | SR | Francis Howell High School | 54.90 | Unverified: |
| 8 | SPROUSE, Tanner | SR | Francis Howell High School | 55.89 | Verified |