| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | AMERSON, Sania | JR | Francis Howell High School | NT | Unverified: |
| 4 | IVORY, Alexandria | FR | Nerinx Hall High School | NT | Unverified: |
| 5 | LEWIS, Ariel | FR | Francis Howell High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BERGOM, Isabel | SO | Nerinx Hall High School | NT | Unverified: |
| 2 | CARAFIOL, Luci | FR | Francis Howell High School | NT | Unverified: |
| 3 | THUET, Allison | FR | Francis Howell High School | NT | Unverified: |
| 4 | SIDWELL, Marissa | JR | Francis Howell High School | NT | Unverified: |
| 5 | JAQUESS, Leiyah | SO | Francis Howell High School | NT | Unverified: |
| 6 | GONZALES, Amari | JR | Francis Howell High School | NT | Unverified: |
| 7 | ZAIDMAN, Naomi | FR | Clayton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROBERTSON, Caroline | FR | Francis Howell High School | NT | Unverified: |
| 2 | WILLETT, Angelica | SR | Francis Howell High School | 1:41.14 | Verified |
| 3 | AGEE, Annelise | JR | Francis Howell High School | 1:20.36 | Verified |
| 4 | CLYNE, Rosemary | FR | Nerinx Hall High School | 1:16.97 | Verified |
| 5 | ANDRES, Maria | SO | Nerinx Hall High School | 1:19.33 | Verified |
| 6 | HOLLINGSWORTH, Jyel | JR | Metro High School | 1:20.94 | Verified |
| 7 | LEWIS, Adley | FR | Metro High School | NT | Unverified: |
| 8 | NGUYEN, Peyton | SR | Francis Howell High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DUNBAR, Isabel | SR | Francis Howell High School | 1:13.82 | Verified |
| 2 | YOST, Avery | SO | Francis Howell High School | 1:11.94 | Verified |
| 3 | MORAN, Sarah | JR | Francis Howell High School | 1:06.64 | Verified |
| 4 | DAUGHTRY, Anna | SO | Affton High School | 1:03.46 | Verified |
| 5 | GRIER, Lucy | SR | Francis Howell High School | 1:05.13 | Verified |
| 6 | COUSINS, Addison | FR | Affton High School | 1:08.37 | Verified |
| 7 | REGA, Morgan | SR | Nerinx Hall High School | 1:12.02 | Verified |
| 8 | BANKS, Lucy | SO | Nerinx Hall High School | 1:16.37 | Verified |