| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clayton High School | C | Bernstein | Cross | Griffey | Trodus | NT | Unverified: |
| 3 | Affton High School | A | Nguyen Buford | Mulat | NT | Verified | ||
| 4 | Clayton High School | A | Stern | Sanders | Ramirez Taylor | Miller | 51.77 | Verified |
| 5 | Francis Howell High School | A | Nelson | Lewis | Waters | Price | 52.75 | Verified |
| 6 | Clayton High School | B | Kabargina | Wolpert | Zimmerman | Zaidman | 57.80 | Unverified: |