| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vianney High School | A | Ivory | Pullam | Abeln | Selsor | 1:38.03 | Verified |
| 2 | Rockwood Summit High School | A | Jackson | Haynes | Oetjen | Kuebee | 1:37.90 | Verified |
| 3 | Union High School | A | Sinclair | Pope | Frazier | Colombo | 1:33.28 | Verified |
| 4 | West Plains High School | A | Stauffer | Miller | Tinsley | Tinsley | 1:29.78 | Verified |
| 5 | Rolla High School | A | Briggs | Stinson | Kearse | Stallworth | 1:32.78 | Verified |
| 6 | St. Clair High School | A | Bartz | Sanders | Furdek | Arndt | 1:35.94 | Verified |
| 7 | Pacific High School | A | Avilez | Thomas | Magsigay | Reeder | 1:37.94 | Verified |
| 8 | Sullivan High School | A | Cain | Beagle | Duran Sabates | York | 1:38.91 | Verified |