| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Sullivan High School | A | Carpenter | Carpenter | McClain | Dace | 2:02.93 | Verified |
| 2 | Union High School | A | Monkman | Sawicki | Spiller | Bockhorst | 1:56.92 | Verified |
| 3 | Washington High School | A | Cragun | Gilliatt | James | Keiser | 1:51.42 | Verified |
| 4 | West Plains High School | A | Oliver | Bowers | King | Dixon | 1:44.91 | Verified |
| 5 | Rockwood Summit High School | A | Turner | Sandler | Stevens | Barafundi | 1:51.02 | Verified |
| 6 | Rolla High School | A | Bales | Martin | Pulliam | Hargis | 1:53.77 | Verified |
| 7 | Pacific High School | A | Edmonds | Dryer | Lay | Blackburn | 1:57.38 | Verified |