| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vianney High School | A | Ivory | Turmelle | Abeln | Lindsey | 3:57.47 | Verified |
| 2 | Sullivan High School | A | Brendel | Beagle | Denney | Duran Sabates | 3:42.79 | Verified |
| 3 | West Plains High School | A | Ingram | Metz | Jacobs | Logue | 3:36.61 | Verified |
| 4 | Union High School | A | Birke | Burke | Swoboda | Pickard | 3:28.05 | Verified |
| 5 | Washington High School | A | Boston | Hendrix | Luttrell | Schiermeier | 3:34.42 | Verified |
| 6 | Rolla High School | A | Briggs | Hamer | Kissinger | Stinson | 3:36.70 | Verified |
| 7 | Rockwood Summit High School | A | Jackson | Hanse | Oetjen | Kuebee | 3:45.04 | Verified |
| 8 | Pacific High School | A | Carrico | Hunkins | Reeder | Stahl | 3:59.82 | Verified |