| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Clair High School | A | Schatzler | Miller | Snell | Cannon | 4:32.83 | Verified |
| 2 | Pacific High School | A | Daniel | Dryer | Murphy | Reed | 4:27.94 | Verified |
| 3 | Rockwood Summit High School | A | Stevens | Cole | Turner | Jackson | 4:16.00 | Verified |
| 4 | West Plains High School | A | Bowers | Oliver | Harris | Dixon | 4:09.47 | Verified |
| 5 | Washington High School | A | Donnelly | James | Keiser | Gilliatt | 4:12.37 | Verified |
| 6 | Rolla High School | A | Pulliam | Ragan | Langley | Bales | 4:19.49 | Verified |
| 7 | Union High School | A | Ahner | Bockhorst | Spurgeon | Brake | 4:29.89 | Verified |
| 8 | Sullivan High School | A | Evans | Mayberry | Brooks | Carpenter | NT | Verified |