| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FROST, Isabella | SR | Rolla High School | 11:18.42 | Verified |
| 2 | ROSSOMANNO, Caterina | SO | Rockwood Summit High School | 11:42.61 | Verified |
| 3 | DONNELLY, Julia | SR | Washington High School | 11:50.78 | Verified |
| 4 | RITTER, Emily | 9 | West Plains High School | 11:55.68 | Verified |
| 5 | SANDERS, Addison | SO | West Plains High School | 12:01.85 | Verified |
| 6 | HIRTZ, Kathryn | JR | Rolla High School | 12:27.33 | Verified |
| 7 | CANNON, Brooklyn | SO | St. Clair High School | 12:29.40 | Verified |
| 8 | JOHANSON, Viola | 9 | Union High School | 12:42.19 | Verified |
| 9 | WHEELER, Leah | SR | Washington High School | 12:44.86 | Verified |
| 10 | CHAPMAN, Kaitlyn | SR | Rockwood Summit High School | 12:55.46 | Verified |
| 11 | JONES, Bella | SO | Pacific High School | 15:20.88 | Verified |
| 12 | LANE, Hanna | SR | Pacific High School | 15:41.99 | Verified |