| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | LIBRE, Hossein | JR | Rolla High School | 23.73 |
| 2 | COLOMBO, Dalton | SO | Union High School | 23.60 |
| 3 | KEARSE, Kennedy | SR | Rolla High School | 22.91 |
| 4 | TINSLEY, Blake | SR | West Plains High School | 22.57 |
| 5 | MILLER, Lincoln | SO | West Plains High School | 22.85 |
| 6 | BLAKE, Casen | 9 | St. Clair High School | 23.12 |
| 7 | PETERSON, Connor | SR | Washington High School | 23.71 |
| 8 | SELSOR, Ian | SO | Vianney High School | 23.93 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PULLAM, Caden | 9 | Vianney High School | 24.76 | Verified |
| 2 | BEKEMEIER, Jensen | SR | St. Clair High School | 24.34 | Verified |
| 3 | PETERSON, Connor | SR | Washington High School | 23.77 | Verified |
| 4 | MILLER, Lincoln | SO | West Plains High School | 22.54 | Verified |
| 5 | KEARSE, Kennedy | SR | Rolla High School | 23.26 | Verified |
| 6 | BLAKE, Casen | 9 | St. Clair High School | 24.10 | Verified |
| 7 | SINCLAIR, Colton | SO | Union High School | 24.35 | Verified |
| 8 | YORK, Robert | SO | Sullivan High School | 24.84 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CAIN, Aidan | JR | Sullivan High School | 24.43 | Verified |
| 2 | COLOMBO, Dalton | SO | Union High School | 24.33 | Verified |
| 3 | LIBRE, Hossein | JR | Rolla High School | 23.59 | Verified |
| 4 | TINSLEY, Blake | SR | West Plains High School | 22.46 | Verified |
| 5 | BOSTON, Landon | JR | Washington High School | 23.34 | Verified |
| 6 | GRIMES, Ryan | JR | Rockwood Summit High School | 24.30 | Verified |
| 7 | SELSOR, Ian | SO | Vianney High School | 24.40 | Verified |
| 8 | BARTON, Mykell | JR | Rockwood Summit High School | NT | Unverified: |