| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Union High School | A | Burke | Herbst | Bailey | Pickard | 8:14.53 | Verified |
| 2 | West Plains High School | A | Gunter | Stiner | Smith | King | 8:31.82 | Verified |
| 3 | Rockwood Summit High School | A | CARUSO | Osterman | Oetjen | Bordner | 8:37.87 | Verified |
| 4 | Rolla High School | A | Lortz | Shenefield | Aguilarleon | Martensen | 8:40.97 | Verified |
| 5 | Washington High School | A | Broadbent | Luttrell | Hewlett | Schiermeier | 8:45.49 | Verified |
| 6 | St. Clair High School | A | James | Slater | Perkins | Kern | 9:03.40 | Verified |
| 7 | Pacific High School | A | Murphy | Robert | Hunkins | Wigger | 9:21.16 | Verified |
| 8 | Vianney High School | A | Hunt | Chou | Loporto | Danforth | 9:34.90 | Verified |
| 9 | Sullivan High School | A | Studdard | Whaley | Summers | Hutchison | 9:59.05 | Verified |