| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PICKARD, Bryson | SR | Union High School | 1:59.65 | Verified |
| 2 | HERBST, William | SR | Union High School | 2:02.65 | Verified |
| 3 | LUTTRELL, Logan | JR | Washington High School | 2:03.38 | Verified |
| 4 | LORTZ, Kyson | SO | Rolla High School | 2:04.42 | Verified |
| 5 | DENNEY, Abram | SO | Sullivan High School | 2:06.01 | Verified |
| 6 | OSTERMAN, Aiden | JR | Rockwood Summit High School | 2:06.26 | Verified |
| 7 | CARUSO, Stephen | SR | Rockwood Summit High School | 2:08.53 | Verified |
| 8 | AGUILARLEON, Nathaniel | SO | Rolla High School | 2:08.93 | Verified |
| 9 | KERN, Aiden | JR | St. Clair High School | 2:10.07 | Verified |
| 10 | SCHIERMEIER, Kellen | SR | Washington High School | 2:11.42 | Verified |
| 11 | JAMES, William | SO | St. Clair High School | 2:14.54 | Verified |
| 12 | HUNKINS, Nick | SR | Pacific High School | 2:14.58 | Verified |
| 13 | SMITH, Trevin | 9 | West Plains High School | 2:15.93 | Verified |
| 14 | ROCHE, Andrew | JR | Vianney High School | 2:16.93 | Verified |
| 15 | WIGGER, Sean | SO | Pacific High School | 2:16.99 | Verified |
| 16 | CHOU, Isaac | SO | Vianney High School | 2:19.44 | Verified |
| 17 | STINER, Hoyt | SO | West Plains High School | 2:21.14 | Verified |
| 18 | WHALEY, Parker | JR | Sullivan High School | 2:34.75 | Verified |