| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KEISER, Josie | 9 | Washington High School | 2:22.06 | Verified |
| 2 | MARTIN, Reese | SO | Rockwood Summit High School | 2:24.02 | Verified |
| 3 | LANGLEY, Kendall | 9 | Rolla High School | 2:26.32 | Verified |
| 4 | THIER, Ella | SR | Rockwood Summit High School | 2:27.92 | Verified |
| 5 | VON BEHREN, Isabella | SO | Washington High School | 2:28.22 | Verified |
| 6 | RAGAN, Bridget | JR | Rolla High School | 2:28.28 | Verified |
| 7 | BRAKE, Kelsey | JR | Union High School | 2:28.51 | Verified |
| 8 | MILLER, Melodi | SR | St. Clair High School | 2:42.26 | Verified |
| 9 | SCHRECK, Cordelia | 9 | Union High School | 2:47.91 | Verified |
| 10 | MCAVOY - KELTZ, LEAH | SR | St. Clair High School | 2:53.12 | Verified |
| 11 | JONES, Ruby | 9 | Pacific High School | 3:03.92 | Verified |
| 12 | CHAMBERS, Gwendolynn | SR | Sullivan High School | 3:38.28 | Verified |
| 13 | BRAUNINGER, Melody | SO | Sullivan High School | 3:40.60 | Verified |