| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | STRONG, Hayden | 9 | West Plains High School | 48.55 | Verified |
| 3 | PITTS, Meshach | SR | Rockwood Summit High School | 46.65 | Verified |
| 4 | CAHILL, James | JR | Rolla High School | 46.05 | Verified |
| 5 | WASHINGTON, Darion | 9 | Union High School | 46.20 | Verified |
| 6 | ZAPOLSKI, Jonathan | JR | Pacific High School | 47.61 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TILLER, Jasahn | 9 | Rockwood Summit High School | 44.96 | Verified |
| 2 | SWOBODA, Evan | SR | Union High School | 41.96 | Verified |
| 3 | WOLF, Sach | JR | Pacific High School | 41.69 | Verified |
| 4 | HURLEY, Lucas | SO | Vianney High School | 39.97 | Verified |
| 5 | STAHL, Thomas | SR | Washington High School | 40.88 | Verified |
| 6 | KISSINGER, Hayden | JR | Rolla High School | 41.83 | Verified |
| 7 | RULO, Landon | 9 | St. Clair High School | 43.67 | Verified |
| 8 | GARRETT, John | SO | Vianney High School | 45.78 | Verified |