| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 4 | CAMPBELL, Alyssa | 9 | Rockwood Summit High School | 52.99 | Verified |
| 5 | NOBLE, Mackenzie | 9 | Rolla High School | 56.22 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SAWICKI, Mikaylyn | SO | Union High School | 52.12 | Verified |
| 2 | SHELTON, Lila | SR | Rockwood Summit High School | 51.02 | Verified |
| 3 | MONKMAN, Camren | SO | Union High School | 49.60 | Verified |
| 4 | OLIVER, Audrey | JR | West Plains High School | 46.65 | Verified |
| 5 | LAY, Alexis | JR | Pacific High School | 49.39 | Verified |
| 6 | SCHATZLER, Cylee | SO | St. Clair High School | 50.60 | Verified |
| 7 | EDMONDS, Arionna | 9 | Pacific High School | 51.25 | Verified |
| 8 | COLENCH, Chelsea | SO | Rolla High School | 52.21 | Verified |