| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | EVANS, Peyton | JR | Sullivan High School | 1:19.48 | Verified |
| 3 | REED, Bailey | SO | Pacific High School | 1:08.06 | Verified |
| 4 | POPLAWSKI, Lilly | JR | Rockwood Summit High School | 1:04.04 | Verified |
| 5 | PULLIAM, Annabella | SO | Rolla High School | 1:04.41 | Verified |
| 6 | MAYBERRY, Breanna | JR | Sullivan High School | 1:08.41 | Verified |
| 7 | SPILLER, Deyonna | 9 | Union High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BALES, Rylie | JR | Rolla High School | 1:03.90 | Verified |
| 2 | VON BEHREN, Isabella | SO | Washington High School | 1:01.47 | Verified |
| 3 | DIXON, Laura | SR | West Plains High School | 1:00.44 | Verified |
| 4 | JACKSON, Barrett | JR | Rockwood Summit High School | 59.42 | Verified |
| 5 | GILLIATT, Abigail | JR | Washington High School | 59.88 | Verified |
| 6 | BOWERS, Katie | SR | West Plains High School | 1:00.74 | Verified |
| 7 | AHNER, Jada | SO | Union High School | 1:02.72 | Verified |
| 8 | DANIEL, Aleyna | SR | Pacific High School | 1:03.98 | Verified |