| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Pacific High School | A | Avilez | Krug | Magsigay | Zapolski | 47.94 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vianney High School | A | Culleton | Battle | Pullam | Ivory | 46.11 | Verified |
| 2 | Sullivan High School | A | Cain | Beagle | Kraus | Duran Sabates | 45.73 | Verified |
| 3 | Washington High School | A | Bartlett | Boston | Peterson | Peterson | 44.96 | Verified |
| 4 | West Plains High School | A | Stauffer | Miller | Tinsley | Miller | 43.06 | Verified |
| 5 | Rolla High School | A | Haas | Stallworth | Kearse | Barr | 44.49 | Verified |
| 6 | St. Clair High School | A | Bartz | Sanders | Bekemeier | Arndt | 45.06 | Verified |
| 7 | Union High School | A | Pope | Frazier | Sinclair | Duffer | 46.01 | Verified |
| 8 | Rockwood Summit High School | A | Jackson | Hanse | Oetjen | Kuebee | 47.13 | Verified |