| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Sullivan High School | A | McClain | Carpenter | Carpenter | Dace | 57.83 | Verified |
| 2 | Pacific High School | A | Huerta | Meyer | Reeder | Adair | NT | Verified |
| 3 | Washington High School | A | Cragun | Guevara | Kroeter | Keiser | 52.48 | Verified |
| 4 | West Plains High School | A | Luck | Bowers | King | Dixon | 49.84 | Verified |
| 5 | Rockwood Summit High School | A | Turner | Fountain | Stevens | Larson | 50.94 | Verified |
| 6 | Rolla High School | A | Obrist | Hargis | Pulliam | Watkins | 53.03 | Verified |
| 7 | St. Clair High School | A | Snell | Mcdonald | McRae | Dunn | 56.72 | Verified |