| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARTENSEN, Cameron | 9 | Rolla High School | 4:25.82 | Verified |
| 2 | SMITH, Ridge | SR | West Plains High School | 4:28.86 | Verified |
| 3 | HERBST, William | SR | Union High School | 4:31.32 | Verified |
| 4 | DENNEY, Abram | SO | Sullivan High School | 4:43.01 | Verified |
| 5 | KING, Carson | SO | West Plains High School | 4:43.29 | Verified |
| 6 | MEYER, Taylor | SO | Union High School | 4:49.47 | Verified |
| 7 | PRATZ, Thomas | SO | Rolla High School | 4:49.58 | Verified |
| 8 | KERN, Aiden | JR | St. Clair High School | 4:54.04 | Verified |
| 9 | PERKINS, Thomas | JR | St. Clair High School | 4:54.15 | Verified |
| 10 | DANFORTH, Vincent | 9 | Vianney High School | 4:54.22 | Verified |
| 11 | BROADBENT, Steven | 9 | Washington High School | 4:57.18 | Verified |
| 12 | ROCHE, Andrew | JR | Vianney High School | 4:58.58 | Verified |
| 13 | STOUT, Dylan | SO | Pacific High School | 4:58.63 | Verified |
| 14 | NUSSER, Nicholas | 9 | Rockwood Summit High School | 5:19.57 | Verified |
| 15 | HEWLETT, Weston | JR | Washington High School | 5:20.22 | Verified |
| 16 | EMMENEGGER, Blake | 9 | Rockwood Summit High School | 5:29.77 | Verified |
| 17 | MURPHY, Joseph | SO | Pacific High School | 5:30.28 | Verified |
| 18 | STUDDARD, Ian | JR | Sullivan High School | 5:52.20 | Verified |