| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LUNA, Adrienne | SR | Cor Jesu Academy | 10:55.87 | Verified |
| 2 | PELLIGREEN, Sophia | SR | Clayton High School | 11:00.79 | Verified |
| 3 | MCKENZIE, Mary | 9 | Cor Jesu Academy | 11:46.45 | Verified |
| 4 | KAISER, Grace | SR | Nerinx Hall High School | 12:49.30 | Verified |
| 5 | CROSS, Isabelle | SR | Clayton High School | 13:00.03 | Verified |
| 6 | HILLMAN, Sophia | SR | Ladue Horton Watkins High School | 13:29.57 | Verified |