| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | John Burroughs High School | A | Spetnagel | Tao | Knispel | Doty | 8:15.01 | Verified |
| 2 | Ladue Horton Watkins High School | A | Gheorghian | Gheorghian | Levine | Raibley | 8:28.99 | Verified |
| 3 | MICDS | A | Dickherber | Fendler | Daus | Williams | 8:59.70 | Verified |
| 4 | Confluence Prep Academy Charter | A | Lance | Dailey-Jones | Green | Gambleton | 9:07.51 | Verified |
| 5 | Clayton High School | A | Zou | Baker | Keller | Hoette | 9:20.40 | Verified |
| 6 | Cardinal Ritter High School | A | ASKEW | Bell | White | Page | 9:23.95 | Verified |