| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 4 | WALKER, Aaliyah | JR | Confluence Prep Academy Charter | 58.26 | Verified |
| 5 | BOYLE, Bry'An | SO | Confluence Prep Academy Charter | 1:14.53 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CROSS, Cesarine | SO | Clayton High School | 54.15 | Verified |
| 2 | CERNY, Maria | SO | Cor Jesu Academy | 53.07 | Verified |
| 3 | DIXON, Laila | JR | University City High School | 50.70 | Verified |
| 4 | MUSIEK, Emma | 9 | Clayton High School | 48.80 | Verified |
| 5 | ROLWES, Bridget | SR | Cor Jesu Academy | 49.53 | Verified |
| 6 | ROGERS, Madison | JR | Ladue Horton Watkins High School | 51.39 | Verified |
| 7 | JACKNEWITZ, Analise | JR | Nerinx Hall High School | 54.01 | Verified |
| 8 | HUGHES, Katherine | JR | Nerinx Hall High School | 55.09 | Verified |