| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KABARGINA, Margaret | 9 | Clayton High School | NT | Unverified: |
| 2 | WOLPERT, Claire | 9 | Clayton High School | 1:13.72 | Verified |
| 3 | BASS, Alyssa | JR | Gateway High School | 1:10.70 | Verified |
| 4 | SIMON, LaTandy | SO | University City High School | 1:06.81 | Verified |
| 5 | GLOVER, Samiyah | SO | Roosevelt High School | 1:10.25 | Verified |
| 6 | MCINTYRE, Chelsie | SO | Collegiate School of Medicine an | 1:11.13 | Verified |
| 7 | HERD-JOHNSON, Asia | 9 | Confluence Prep Academy Charter | 1:16.99 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GUY, Kimora | JR | Confluence Prep Academy Charter | 1:05.31 | Verified |
| 2 | BROWN, Tiara | JR | Gateway High School | 1:02.77 | Verified |
| 3 | WHITE, Makayla | SR | Ladue Horton Watkins High School | 59.07 | Verified |
| 4 | BRINKER, Delaney | SO | Ladue Horton Watkins High School | 57.42 | Verified |
| 5 | MEDINA, Luciana | JR | Nerinx Hall High School | 57.47 | Verified |
| 6 | BRECKENRIDGE, Dylan | 9 | Cor Jesu Academy | 1:02.56 | Verified |
| 7 | BOGNAR, Ava | 9 | Cor Jesu Academy | 1:04.88 | Verified |
| 8 | HENNESSEY, Elianna | 9 | Nerinx Hall High School | 1:06.55 | Verified |