| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Gateway High School | A | Nevils | Carothers | Brown | Bass | 53.09 | Verified |
| 3 | Clayton High School | A | Ramirez Taylor | Miller | Stern | Sanders | 51.33 | Verified |
| 4 | Ladue Horton Watkins High School | A | Ray | Buxner | Murray | White | 48.83 | Verified |
| 5 | Confluence Prep Academy Charter | A | Guy | Huntley | Daniels | Waters | 51.05 | Verified |
| 6 | Cor Jesu Academy | A | Stoeckel | Jacoby | Lorms | Graham | 52.69 | Verified |
| 7 | Nerinx Hall High School | A | Hughes | Johnson | Gallagher | Albillo | DNS | Verified |