| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cor Jesu Academy | A | Stirton | McKenzie | Luna | Luna | 9:46.09 | Verified |
| 2 | Nerinx Hall High School | A | Stann | Kaiser | Jacknewitz | Medina | 9:58.67 | Verified |
| 3 | Clayton High School | A | Caroline Welch | Cross | Musiek | Erdmann | 10:17.99 | Verified |
| 4 | Ladue Horton Watkins High School | A | Hillman | Hillman | Tsygankov | Raibley | 11:48.73 | Verified |