| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HAYS, Ella | JR | Monroe City High School | 6:10.65 | Verified |
| 2 | BREWER, Gabrielle | SR | Kirksville High School | 6:11.48 | Verified |
| 3 | STAHL, Kaycie | JR | Highland High School | 6:20.24 | Verified |
| 4 | JOHNSON, Avery | SO | Monroe City High School | 6:23.39 | Verified |
| 5 | OLSEN, Rylee | JR | Keokuk High School | 6:32.72 | Verified |
| 6 | JOHNSON, Mia | JR | Clark County High School | 6:34.65 | Verified |
| 7 | SUBLETTE, Mallory | SR | Palmyra High School | 6:35.32 | Verified |
| 8 | LITTLE, TRINITY | SO | Clark County High School | 6:38.35 | Verified |
| 9 | WEIMAN, Ava | JR | Palmyra High School | 6:54.13 | Verified |
| 10 | STUPAVSKY, Isabella | SR | Monroe City High School | 6:58.52 | Verified |
| 11 | RUFENER, Tadara | SR | South Shelby High School | 7:01.26 | Verified |
| 12 | BEHNE, Maren | SR | Kirksville High School | 7:04.65 | Verified |
| 13 | BUCKMAN, Shelby | SO | South Shelby High School | 7:10.68 | Verified |
| 14 | OLSEN, Jacie | FR | Keokuk High School | 7:25.00 | Unverified: |
| 15 | AVERY, Phoenix | FR | South Shelby High School | 7:53.82 | Verified |
| 16 | BREWER, Claire | FR | Kirksville High School | NT | Unverified: |
| 17 | HODGES, Makenna | SO | Clark County High School | NT | Unverified: |