| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Scotland County High School | A | HAMLIN | Bishop | ANDERS | Holland | 2:10.67 | Unverified: |
| 2 | Keokuk High School | A | Stewart | Stewart | Crew | Sistern | 2:02.29 | Unverified: |
| 3 | South Shelby High School | A | Eleazarraraz | Gaines | Nelson | Wilt | 1:56.19 | Verified |
| 4 | Palmyra High School | A | Redd | Loman | McBride | White | 1:54.86 | Verified |
| 5 | Highland High School | A | Sharpe | Keith | Bringer | Reed | 1:55.72 | Verified |
| 6 | Kirksville High School | A | Baumgartner | Herbst | Butler | Harris | 1:57.31 | Verified |
| 7 | Monroe City High School | A | Clark | Flick | Martin | Lehenbauer | 2:05.82 | Verified |
| 8 | Clark County High School | A | Lovell | IRVIN | West | Veloso | NT | Unverified: |