| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Palmyra High School | A | Harper | Redd | Reid | Sheppard | NT | Unverified: |
| 2 | Keokuk High School | A | Glasgow | Burton | Gibbs | Claus | 4:03.28 | Unverified: |
| 3 | Kirksville High School | A | Mendez | Novencido | Adams | Bedford | 3:55.61 | Verified |
| 4 | South Shelby High School | A | Hammond | Elsen | Wiseman | Wilt | 3:39.73 | Verified |
| 5 | Monroe City High School | A | Karr | Wheeler | Lehenbauer | Woolen | 3:49.81 | Verified |
| 6 | Clark County High School | A | SHINN | Day | Westercamp | WHEELER | 3:55.74 | Verified |
| 7 | Highland High School | A | Caudill | Evans | Hinton | Stegeman | 4:08.92 | Verified |