| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Monroe City High School | A | Clark | Flick | Hathaway | Martin | 4:50.54 | Verified |
| 2 | Clark County High School | A | Plenge | Little | WEAVER | YATES | 4:44.01 | Unverified: Davis County Boarder Battle |
| 3 | Kirksville High School | A | Herbst | Thomas | Brewer | Harris | 4:40.96 | Verified |
| 4 | South Shelby High School | A | Eleazarraraz | Mitchell | Roush | McWilliams | 4:27.39 | Verified |
| 5 | Palmyra High School | A | Sheputis | Leon | Loman | White | 4:33.15 | Unverified: |
| 6 | Highland High School | A | Reed | Keith | Abell | Sharpe | 4:42.60 | Verified |
| 7 | Keokuk High School | A | Sistern | Haage | Gobart | Takorbisong | 4:50.03 | Unverified: |