| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Monroe City High School | A | Lehenbauer | Richardson | Hunter | Lorenson | 9:21.16 | Verified |
| 2 | Marion County High School | A | Britt | Schluckebier | Tuley | Wood | 9:28.57 | Verified |
| 3 | Clark County High School | A | Hurt | Wardlow | McDaniel | Day | 9:38.82 | Verified |
| 4 | Clark County High School | B | King | Thompson | Roach | Schuetz | 9:38.82 | Verified |
| 5 | Palmyra High School | A | Bier | Bross | Lovegreen | Jurado | 9:48.23 | Verified |
| 6 | Kirksville High School | A | GRISSOM | Johnson | James | Schutter | 9:55.79 | Verified |
| 7 | Highland High School | A | Self | Caudill | Evans | Clow | 10:08.69 | Verified |