| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WHITE, Mandy | JR | Green City High School | 34.38 | Verified |
| 4 | CRIST, Bailie | FR | Highland High School | 32.76 | Verified |
| 5 | SLATER, Sophia | FR | Canton High School | 33.66 | Verified |
| 6 | MYERS, Mylee | FR | Marion County High School | 37.17 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STEWART, Aaliyah | SO | Keokuk High School | 31.88 | Unverified: |
| 2 | ANDERS, EMMA | JR | Scotland County High School | 31.07 | Verified |
| 3 | CLARK, Jaelynn | JR | Monroe City High School | 30.47 | Verified |
| 4 | TASCO, Amelia | SR | Highland High School | 29.92 | Verified |
| 5 | WILT, Madison | SO | South Shelby High School | 30.30 | Unverified: Monroe City HS Invitational |
| 6 | TAKORBISONG, Kelly | SO | Keokuk High School | 30.54 | Unverified: |
| 7 | BOCK, Maya | FR | Marion County High School | 31.16 | Verified |
| 8 | MARTIN, Mya | FR | Monroe City High School | 32.42 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | YATES, KAYLIE | SO | Clark County High School | 29.46 | Verified |
| 2 | ROUSH, Elizabelle | SO | South Shelby High School | 29.07 | Verified |
| 3 | CLAY, Nariah | SR | Canton High School | 28.15 | Verified |
| 4 | WHITE, Taytum | JR | Palmyra High School | 27.12 | Verified |
| 5 | PLENGE, Abby | SR | Clark County High School | 27.34 | Verified |
| 6 | HOLLAND, Charlee | FR | Scotland County High School | 28.25 | Verified |
| 7 | THOMAS, Maya | SR | Kirksville High School | 29.18 | Verified |
| 8 | BUTLER, Jaliyah | JR | Kirksville High School | 29.67 | Verified |