| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WHEELER, Jackson | SR | Monroe City High School | 2:10.26 | Verified |
| 2 | SCHUTTER, Justin | FR | Kirksville High School | 2:14.27 | Verified |
| 3 | DAY, Austin | SO | Clark County High School | 2:21.17 | Verified |
| 4 | EVANS, Charlie | SO | Highland High School | 2:23.02 | Verified |
| 5 | MOORE, Titus | SO | South Shelby High School | 2:23.43 | Verified |
| 6 | LITTRELL, Ivan | FR | Green City High School | 2:24.06 | Verified |
| 7 | SCHLUCKEBIER, Rheo | SO | Marion County High School | 2:28.00 | Verified |
| 8 | WOOD, Mason | SO | Marion County High School | 2:28.00 | Verified |
| 9 | BERHORST, Dale | SO | Canton High School | 2:28.00 | Verified |
| 10 | JURADO, Landon | FR | Palmyra High School | 2:29.25 | Verified |
| 11 | HUNTER, Aidan | SO | Monroe City High School | 2:29.86 | Verified |
| 12 | WARDLOW, Isaac | SO | Clark County High School | 2:31.14 | Verified |
| 13 | GRISSOM, CALEB | FR | Kirksville High School | 2:31.80 | Verified |
| 14 | LOVEGREEN, Ashton | FR | Palmyra High School | 2:32.32 | Verified |
| 15 | BRITT, Porter | SO | Marion County High School | 2:34.11 | Verified |
| 16 | SEATON, Cooper | FR | South Shelby High School | 2:34.76 | Verified |
| 17 | GIBBS, Elijah | JR | Keokuk High School | 2:35.11 | Unverified: |
| 18 | DELPERDANG, Nolen | SO | Keokuk High School | 2:40.71 | Unverified: |
| 19 | SELF, Dade | FR | Highland High School | 2:45.07 | Verified |
| 20 | SMITH, Carter | FR | Scotland County High School | 2:59.80 | Verified |
| 21 | JAMES, Sean | FR | Kirksville High School | NT | Unverified: |
| 22 | SCHUETZ, Gavin | SO | Clark County High School | NT | Unverified: |