| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLAY, Nariah | SR | Canton High School | 2:23.70 | Verified |
| 2 | SHEPUTIS, Laurin | JR | Palmyra High School | 2:26.85 | Verified |
| 3 | STAHL, Kaycie | JR | Highland High School | 2:43.04 | Verified |
| 4 | BREWER, Gabrielle | SR | Kirksville High School | 2:43.60 | Verified |
| 5 | WEAVER, MAGGIE | JR | Clark County High School | 2:50.79 | Verified |
| 6 | HAYS, Ella | JR | Monroe City High School | 2:54.57 | Verified |
| 7 | JOHNSON, Avery | SO | Monroe City High School | 2:55.39 | Verified |
| 8 | LEON, Sandra | FR | Palmyra High School | 2:56.86 | Verified |
| 9 | LITTLE, Tianna | FR | Clark County High School | 2:59.47 | Unverified: Davis County Boarder Battle |
| 10 | BUCKMAN, Shelby | SO | South Shelby High School | 3:01.01 | Verified |
| 11 | HODGES, Makenna | SO | Clark County High School | 3:06.91 | Verified |
| 12 | OLSEN, Rylee | JR | Keokuk High School | 3:10.54 | Unverified: |
| 13 | ORTEGA, Milly | FR | Green City High School | 3:11.68 | Verified |
| 14 | BREWER, Claire | FR | Kirksville High School | 3:18.41 | Verified |
| 15 | AVERY, Phoenix | FR | South Shelby High School | 3:18.98 | Verified |
| 16 | AMES, Kenadi | FR | Keokuk High School | 3:23.48 | Unverified: |
| 17 | OLSEN, Jacie | FR | Keokuk High School | 3:23.92 | Unverified: |
| 18 | HAYS, Marissa | FR | Monroe City High School | 3:30.17 | Verified |
| 19 | GILL, Somer | JR | South Shelby High School | 3:43.90 | Verified |
| 20 | VICE, Faith | FR | Kirksville High School | NT | Unverified: |
| 21 | LEE, Alyssa | FR | Marion County High School | NT | Unverified: |