| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MILLER, Payton | SO | Scotland County High School | 1:05.17 | Verified |
| 2 | BEHNE, Maren | SR | Kirksville High School | 1:03.03 | Verified |
| 3 | RUBA, Sadie | SR | Green City High School | 59.92 | Verified |
| 4 | NEVES, LUNDYN | JR | Clark County High School | 57.62 | Verified |
| 5 | HATHAWAY, Kennedy | FR | Marion County High School | 58.51 | Verified |
| 6 | HOLLENDER, Brinley | JR | Clark County High School | 1:01.98 | Verified |
| 7 | GOBART, Lily | SR | Keokuk High School | 1:03.54 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FOSS, Gracee | SO | Monroe City High School | 54.39 | Verified |
| 2 | MITCHELL, Kamryn | JR | South Shelby High School | 51.44 | Unverified: South Shelby Open |
| 3 | VOSS, Tenlee | SR | Palmyra High School | 49.96 | Unverified: Monroe City Invitational |
| 4 | HERBST, Jersey | JR | Kirksville High School | 46.64 | Verified |
| 5 | YOUNGBLOOD, Audri | SO | Monroe City High School | 48.26 | Verified |
| 6 | BRINGER, Ansley | SR | Highland High School | 50.78 | Verified |
| 7 | BISHOP, Paige | SR | Scotland County High School | 53.70 | Verified |
| 8 | STEWART, Layonna | SO | Keokuk High School | 56.39 | Verified |