| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | FLICK, Rylee | FR | Monroe City High School | NT | Unverified: |
| 4 | MYERS, Mylee | FR | Marion County High School | 1:29.40 | Verified |
| 5 | WAGNER, Sharli | SO | Marion County High School | 1:33.81 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | TIBBITS, Halli | FR | Keokuk High School | 1:16.00 | Unverified: |
| 3 | TRIPLETT, Janie | FR | Palmyra High School | 1:14.23 | Verified |
| 4 | DOWNEN, Phebe | SR | Kirksville High School | 1:13.86 | Verified |
| 5 | HAAGE, Reagan | SO | Keokuk High School | 1:14.00 | Unverified: |
| 6 | ABELL, Addy | FR | Highland High School | 1:14.72 | Verified |
| 7 | MARTIN, Mya | FR | Monroe City High School | 1:16.25 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GAINES, Hannah | FR | South Shelby High School | 1:08.78 | Verified |
| 2 | YATES, KAYLIE | SO | Clark County High School | 1:07.13 | Verified |
| 3 | LOMAN, Alaina | JR | Palmyra High School | 1:03.15 | Verified |
| 4 | PLENGE, Abby | SR | Clark County High School | 1:00.73 | Verified |
| 5 | SHARPE, Chevie | FR | Highland High School | 1:02.51 | Verified |
| 6 | HARRIS, Mya | FR | Kirksville High School | 1:04.49 | Verified |
| 7 | WILT, Madison | SO | South Shelby High School | 1:08.77 | Verified |
| 8 | HUDNUT, Laken | FR | Canton High School | 1:11.50 | Verified |