| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Highland High School | A | Stegeman | Hinton | Moore | Gaus | 53.09 | Unverified: |
| 4 | Monroe City High School | A | Jones | Karr | Warden | McFall | 51.56 | Verified |
| 5 | Scotland County High School | A | Shaffer | Austin | Mallett | Myers | 52.05 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Keokuk High School | A | Burton | Hoard | Hayner | Arntzen | 50.77 | Unverified: |
| 3 | Kirksville High School | A | Omehele | Mendez | Novencido | Novencido | 48.22 | Verified |
| 4 | Clark County High School | A | SHINN | DAVIDSON | WHEELER | HAMNER | 46.55 | Verified |
| 5 | South Shelby High School | A | Moellering | Wilt | Wiseman | Hammond | 47.09 | Verified |
| 6 | Palmyra High School | A | Hicks | Pennewell | Redd | Bier | 49.66 | Verified |
| 7 | Green City High School | A | Crist | Forest | Johnson | Navis | 51.04 | Verified |