| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Clark County High School | A | MCKAY | Lovell | NEVES | Veloso | NT | Unverified: |
| 4 | Scotland County High School | A | HAMLIN | Bishop | Robinson | Holland | 59.61 | Unverified: |
| 5 | Marion County High School | A | Bock | Hathaway | Lee | Myers | 1:00.63 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Green City High School | A | Gordon | Hatcher | Helton | Jeffries | 57.43 | Verified |
| 2 | Keokuk High School | A | Stewart | Stewart | Crew | Takorbisong | 56.78 | Unverified: |
| 3 | Highland High School | A | Sharpe | Keith | Bringer | Reed | 54.80 | Verified |
| 4 | Palmyra High School | A | Redd | Voss | McBride | White | 53.25 | Verified |
| 5 | South Shelby High School | A | McWilliams | Mitchell | Eleazarraraz | Roush | 54.34 | Verified |
| 6 | Kirksville High School | A | Baumgartner | Downen | Butler | Thomas | 56.51 | Verified |
| 7 | Monroe City High School | A | Foss | Lehenbauer | Clark | Hays | 57.11 | Verified |