| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SCHUTTER, Justin | FR | Kirksville High School | 4:52.69 | Verified |
| 2 | WRIGHT, Levi | SO | Marion County High School | 4:56.52 | Verified |
| 3 | WHEELER, Jackson | SR | Monroe City High School | 4:59.29 | Verified |
| 4 | BROSS, Connor | SR | Palmyra High School | 5:04.64 | Verified |
| 5 | MCDANIEL, Mason | FR | Clark County High School | 5:13.00 | Unverified: Davis County Boarder Battle |
| 6 | HURT, Kindric | SO | Clark County High School | 5:16.20 | Unverified: Davis County Boarder Battle |
| 7 | BRITT, Porter | SO | Marion County High School | 5:17.24 | Verified |
| 8 | MOORE, Titus | SO | South Shelby High School | 5:18.52 | Verified |
| 9 | SEATON, Cooper | FR | South Shelby High School | 5:22.26 | Verified |
| 10 | CLOW, Ethan | JR | Highland High School | 5:23.11 | Verified |
| 11 | LITTRELL, Ivan | FR | Green City High School | 5:31.89 | Verified |
| 12 | RICHARDSON, Alston | FR | Monroe City High School | 5:39.97 | Verified |
| 13 | LOVEGREEN, Ashton | FR | Palmyra High School | 5:45.75 | Verified |
| 14 | CAUDILL, Nathan | FR | Highland High School | 5:47.63 | Verified |
| 15 | HUNTER, Aidan | SO | Monroe City High School | 5:48.17 | Verified |
| 16 | GIBBS, Elijah | JR | Keokuk High School | 5:52.49 | Verified |
| 17 | MASON KIEFABER, OWEN | SR | Palmyra High School | 5:55.45 | Verified |
| 18 | STERUP, Daniel | JR | Kirksville High School | 5:58.49 | Verified |
| 19 | WARDLOW, Isaac | SO | Clark County High School | NT | Unverified: |
| 20 | SHAFFER, Quinton | SR | Scotland County High School | NT | Unverified: |
| 21 | MULLANIX, Gabriel | JR | Kirksville High School | NT | Unverified: |