| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Smith-Cotton High School | A | King | Ladd | Matacuaubaldo | Zalpa | 3:44.70 | Verified |
| 2 | Battle High School | A | Owusu | Mugisha | Karara | Lakes | 3:38.31 | Verified |
| 3 | Capital City High School | A | Harrison | Schofield | Davis | Grieve | 3:29.73 | Verified |
| 4 | Helias Catholic High School | A | Fisher | Knernschield | Wilson | Malmstrom | 3:29.50 | Verified |
| 5 | Rock Bridge High School | A | Seamon | Kemey | Hood | Holt | 3:29.52 | Verified |
| 6 | Hickman High School | A | DiBlasi | Keys | Wright | Zink | 3:31.21 | Verified |
| 7 | Jefferson City High School | A | Jordan | Stumbaugh | Wilson | Burns | 3:44.46 | Verified |