| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Smith-Cotton High School | A | Askerov | Gray | Clark | Young | NT | Verified |
| 2 | Capital City High School | A | Bonchonsky | Macias | Breuer | McRoberts | 3:40.73 | Unverified: |
| 3 | Rock Bridge High School | A | Scott | Hicklin | Chinikoski | Hockman | 3:39.52 | Unverified: |
| 4 | Helias Catholic High School | A | Crisostomo | Swan | Schatzer | Jerozal | 3:29.50 | Verified |
| 5 | Hickman High School | A | Brown | Fisher | Frederick | Renoe | 3:38.21 | Unverified: |
| 6 | Battle High School | A | Dennison | Rice | Alderton | McCray | 3:40.00 | Unverified: |
| 7 | Jefferson City High School | A | Center | Johnson | Rukaria | Fontenot | NT | Verified |