| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Smith-Cotton High School | A | Newkirk | Spellmeyer | Kensinger | Kuehner | 4:58.23 | Verified |
| 2 | Hickman High School | A | Leigh | Maland | Stemmons | Eastman | 4:23.52 | Verified |
| 3 | Helias Catholic High School | A | Verslues | Moellers | Dollar | Harrison | 4:14.82 | Verified |
| 4 | Rock Bridge High School | A | Ford | Miller | Parsons | Crane | 4:04.66 | Verified |
| 5 | Jefferson City High School | A | Pemberton | Galbreath | Schonhardt | Morris | 4:10.66 | Verified |
| 6 | Battle High School | A | Johnson | Anye | Gaines | Butler | 4:16.76 | Verified |
| 7 | Capital City High School | A | Sterrett | Robben | Pistel | Hess | 4:23.91 | Verified |