| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Hickman High School | A | Chang | Cole | Hofeditz | Redmond-Lilly | 4:40.52 | Unverified: |
| 3 | Battle High School | A | Glay | May | Carter | Schultz | NT | Unverified: |
| 4 | Helias Catholic High School | A | Broker | Lock | French | Delk | 4:14.82 | Verified |
| 5 | Capital City High School | A | Kerschner | Mays | Ndegwa | Lutz-Jenkins | 4:23.91 | Verified |
| 6 | Rock Bridge High School | A | Crane | Wheeler | Gillis | Ireland | NT | Unverified: |
| 7 | Smith-Cotton High School | A | Holiway | Popova | Brosch | Johnson | NT | Verified |