| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SEXTEN, Morgan | SR | Rock Bridge High School | 11:56.70 | Verified |
| 2 | RICHARDSON, Claire | FR | Rock Bridge High School | 12:08.08 | Verified |
| 3 | EASTMAN, Ellie | SO | Hickman High School | 12:16.26 | Verified |
| 4 | BUKER, Danielle | SR | Helias Catholic High School | 12:24.02 | Verified |
| 5 | KIRBY, Sarah | SR | Capital City High School | 12:32.85 | Verified |
| 6 | MATHEWS, Lauren | SO | Helias Catholic High School | 12:41.30 | Verified |
| 7 | SCHNIPPER, Jenna | FR | Jefferson City High School | 13:02.03 | Verified |
| 8 | JONES, Adelaide | JR | Jefferson City High School | 13:15.91 | Verified |
| 9 | GONZALEZ, Daniela | SO | Smith-Cotton High School | 13:35.80 | Verified |