| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Capital City High School | A | Davis | Grieve | Noble | Voss | 7:56.45 | Verified |
| 2 | Rock Bridge High School | A | Welty | Lee | Baehr | Kemey | 8:01.61 | Verified |
| 3 | Helias Catholic High School | A | Wilson | Ammons | Benne | Doolittle | 8:15.25 | Verified |
| 4 | Hickman High School | A | Brinkman | Hogan | Keys | Wise | 8:36.89 | Verified |
| 5 | Jefferson City High School | A | Stumbaugh | Waite | Gerbes | Gerbes | 9:02.64 | Verified |
| 6 | Smith-Cotton High School | A | King | Matacuaubaldo | Wilwohl | Zalpa | 9:07.00 | Verified |