| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BRISCOE, Lakira | FR | Hickman High School | 27.94 | Verified |
| 3 | GERRISH, Addyelin | SO | Rock Bridge High School | 27.63 | Verified |
| 4 | STRAYHORN, Kalie | SR | Capital City High School | 26.89 | Verified |
| 5 | CRANE, Sunday | SR | Rock Bridge High School | 27.06 | Verified |
| 6 | OKPODIGHE, Emerald | JR | Jefferson City High School | 27.66 | Verified |
| 7 | BELL, Dominique | FR | Smith-Cotton High School | 28.34 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | POWELL, Sophia | SR | Smith-Cotton High School | 26.70 | Verified |
| 2 | STRUEMPH, Isabelle | JR | Helias Catholic High School | 26.20 | Verified |
| 3 | ANYE, Sarah | SR | Battle High School | 26.03 | Verified |
| 4 | EDWARDS, Mikah | SR | Helias Catholic High School | 24.86 | Verified |
| 5 | PETERSON, Athena | SO | Hickman High School | 25.67 | Verified |
| 6 | STERRETT, Tyrica | SO | Capital City High School | 26.12 | Verified |
| 7 | JONES, Ja'Kiyra | FR | Battle High School | 26.39 | Verified |
| 8 | MORRIS, Seleena | SR | Jefferson City High School | 26.86 | Verified |