| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WRAY, Naiya | JR | Hickman High School | 31.63 | Verified |
| 4 | BIBBS, Salaysia | FR | Capital City High School | 31.48 | Verified |
| 5 | O'DONNELL, Elliot | SO | Jefferson City High School | 31.59 | Verified |
| 6 | LOCK, Andrea | FR | Helias Catholic High School | 31.82 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SANTIAGO, Araya | FR | Capital City High School | 30.86 | Verified |
| 2 | HARDEN, Shanaya | FR | Jefferson City High School | 30.08 | Verified |
| 3 | SZYDLOWSKI, Alexis | FR | Rock Bridge High School | 29.43 | Verified |
| 4 | SCHULTZ, Brianna | SR | Battle High School | 28.36 | Verified |
| 5 | FISHER, Shaniya | FR | Rock Bridge High School | 29.40 | Verified |
| 6 | MAY, Ky’Anna | JR | Battle High School | 29.93 | Verified |
| 7 | ZAPIEN, Aimarie | FR | Hickman High School | 30.34 | Verified |
| 8 | FRENCH, Marlee | FR | Helias Catholic High School | 31.28 | Verified |