| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GRIEVE, Keion | JR | Capital City High School | 1:51.25 | Verified |
| 2 | HAUSER, Andrew | JR | Rock Bridge High School | 1:56.91 | Verified |
| 3 | KEMEY, Ian | SR | Rock Bridge High School | 1:57.25 | Verified |
| 4 | WILSON, Ethan | SR | Helias Catholic High School | 1:57.86 | Verified |
| 5 | SCHOFIELD, Brock | SR | Capital City High School | 2:00.80 | Verified |
| 6 | KEYS, Sean | JR | Hickman High School | 2:04.04 | Verified |
| 7 | REED, Marshall | SR | Hickman High School | 2:05.45 | Verified |
| 8 | AMMONS, Nick | JR | Helias Catholic High School | 2:07.34 | Verified |
| 9 | TUYISENGE, Eric | SR | Battle High School | 2:12.10 | Verified |
| 10 | RUKARIA, Austin | JR | Jefferson City High School | 2:16.93 | Verified |
| 11 | MATACUAUBALDO, Juan | SO | Smith-Cotton High School | 2:17.07 | Verified |
| 12 | WAITE, Lucas | FR | Jefferson City High School | 2:20.37 | Verified |
| 13 | KING, Morgan | SR | Smith-Cotton High School | 2:21.89 | Verified |
| 14 | MUGISHA, Habimana | SR | Battle High School | 2:24.96 | Verified |