| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FORD, Carolyn | SR | Rock Bridge High School | 2:14.18 | Verified |
| 2 | WALKER, Mae | FR | Rock Bridge High School | 2:23.30 | Verified |
| 3 | VERSLUES, Lauren | SO | Helias Catholic High School | 2:26.98 | Verified |
| 4 | ROBBEN, Yumia | SR | Capital City High School | 2:29.07 | Verified |
| 5 | BUKER, Danielle | SR | Helias Catholic High School | 2:33.57 | Verified |
| 6 | HARRIS, Allison | SR | Battle High School | 2:36.60 | Verified |
| 7 | KEMNA, Kari | SO | Capital City High School | 2:41.48 | Verified |
| 8 | KINSEY, Ella | SO | Jefferson City High School | 2:41.82 | Verified |
| 9 | CARRICO, Lindsey | FR | Smith-Cotton High School | 2:41.97 | Verified |
| 10 | SMITH, Victoria | SO | Jefferson City High School | 2:45.54 | Verified |
| 11 | HANER, Avery | JR | Battle High School | 2:49.45 | Verified |
| 12 | KUEHNER, Julie | SO | Smith-Cotton High School | 2:55.32 | Verified |