| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 13 | CRANE, Amelie | SO | Rock Bridge High School | 2:29.99 | Verified |
| 14 | GILLIS, Peyton | JR | Rock Bridge High School | 2:31.41 | Verified |
| 15 | O'LAUGHLIN, Zoe | FR | Hickman High School | 2:38.93 | Verified |
| 16 | WEBER, Iris | FR | Hickman High School | 2:49.97 | Verified |
| 17 | BROKER, Erin | FR | Helias Catholic High School | 2:53.45 | Verified |
| 18 | COLEMAN, Jayden | SO | Battle High School | 3:03.41 | Verified |
| 19 | SUTHOFF, Sydney | FR | Helias Catholic High School | 3:08.05 | Verified |
| 20 | CASTRO, Ezra | FR | Battle High School | 3:09.24 | Verified |
| 21 | CHEN, Ria | SR | Capital City High School | 3:09.54 | Verified |
| 22 | BERNSKOETTER, Addison | FR | Capital City High School | 3:19.07 | Verified |
| 23 | BROSCH, Emma | SO | Smith-Cotton High School | 3:29.18 | Verified |