| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | LYSON, Mikayla | FR | Rock Bridge High School | 54.68 | Verified |
| 4 | OKPODIGHE, Emerald | JR | Jefferson City High School | 52.99 | Verified |
| 5 | HILL, Carmen | SO | Capital City High School | 53.42 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DOMEIER, Isabelle | SO | Hickman High School | 52.10 | Unverified: |
| 2 | CRISOSTOMO, Daelyn | FR | Helias Catholic High School | 51.05 | Verified |
| 3 | GALBREATH, Cheria | JR | Jefferson City High School | 46.59 | Verified |
| 4 | PARSONS, Grace | SR | Rock Bridge High School | 44.65 | Verified |
| 5 | PETERSON, Athena | SO | Hickman High School | 45.31 | Verified |
| 6 | BUTLER, Jordyn | SR | Battle High School | 48.96 | Verified |
| 7 | SHEWMAKER, Madilyn | FR | Capital City High School | 51.78 | Verified |
| 8 | NEWKIRK, Natali | SO | Smith-Cotton High School | 52.53 | Verified |