| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 7 | Capital City High School | A | Walker | Whitaker | Sharp | Golden | 7:56.45 | Verified |
| 8 | Helias Catholic High School | A | Voegeli | Wieberg | Borgmeyer | Loesch | 8:15.25 | Verified |
| 9 | Hickman High School | A | Current | Lee | Villasana | Waller | 8:50.89 | Unverified: |
| 10 | Rock Bridge High School | A | Tiemeyer | Canlas | Mettu | Bredehoeft | 8:51.61 | Unverified: |
| 11 | Jefferson City High School | A | Conger | Reeves | Woodruff | Dawson | 9:02.64 | Verified |
| 12 | Smith-Cotton High School | A | Gonzalez | King | Rebato | Ellison | 9:15.00 | Unverified: |
| 13 | Battle High School | A | Bradshaw | Bradshaw | Hall | Buxton | 9:20.00 | Unverified: |